(R)-4-propyl-dihydro-furan-2-One CAS 63095-51-2

(R)-4-propyl-dihydrofuran-2-one is an intermediat CAS number is 63095-51-2, which is an important key intermediate . His synthetic methods are mainly divided into chemical synthesis and enzymatic catalysis: The first one is prepared by reacting (R)-epichlorohydrin with diphenyl malonate to obtain phenyl 2-oxo-3-oxabicyclo[3.1.0]hexane-1-carboxylate. And then reacted with ethylmagnesium bromide to obtain 2-oxo-4-propyl-tetrahydrofuran-3-carboxylic acid phenyl ester, which is decarboxylated at high temperature to obtain (R)-4-propyl-di Hydrofuran-2-one cas 63059-51-2. This method is costly. The second, (1) the double-digested expression vector pET-30a was ligated with the synthetic target gene by T4 ligase overnight, and 10 uL of the ligation product was added to 100 uL of Rosetta (DE3) competent cells, and placed on ice 30 min, heat shock at 42 ℃ for 60 sec. Place on ice for 2 min. The pre-warmed 0.45 mL LB medium was added and incubated at 37 ℃ for 1 h at 220 ℃. 200 uL of…